Radio Broadcasting Preservation Act of 2000 - Directs the Federal Communications Commission (FCC) to modify its rules authorizing the operation of low-power FM radio stations to: (1) prescribe minimum distance separations for third-adjacent channels (as well as co-channels and first- and second-adjacent channels); and (2) prohibit any applicant from obtaining a low-power FM license if such applicant has engaged in the unlicensed operation of any radio station in violation of FCC radio licensing requirements.

Prohibits the FCC, without specific authorization by Congress, from: (1) eliminating or reducing such minimum distance separations for third-adjacent channels; or (2) extending the eligibility for low-power FM stations beyond those organizations and entities proposed in MM Docket No. 99-25.

Invalidates any previously issued low-power FM station license that does not comply with such rule modifications.

Directs the FCC to conduct an experimental program to test whether low- power FM stations will result in harmful interference to existing FM radio stations if such stations are not subject to the minimum distance separation requirements. Requires the FCC to: (1) publish test results and allow an opportunity for public comment; and (2) report test results and FCC recommendations on reducing or eliminating minimum distance standards to specified congressional committees.